To detect genetic variations, inter simple sequence repeat (ISSR) markers are commonly utilised. ISSR markers were used to determine genetic diversity in 13 chickpea accessions, both cultivated and wild. The pentanucleotide repeat primer UBC-879 generated the best amplification patterns of all the anchored ISSR primers tested. In total, 150 bands were amplified in the 100-2000 bps molecular weight range, yielding an average of 21.4 bands per primer and 1.64 bands per primer per genotype. Amplification is least with the repeats (GA)8C, (AG)8YT, (GA)8YC, (AG)8C, (GTT)6, and (GT)8YC.
Author (S) Details
Neha Gupta
School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, Madhya Pradesh, India.
Sameer Suresh Bhagyawant
School of Studies in Biotechnology, Jiwaji University, Gwalior 474011, Madhya Pradesh, India.
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